Shared Research Facilities and Equipment

The Share Resource serves Duke and non-Duke researchers with Illumina (Hiseq 2000/2500 and MiSeq), Ion Torrent (PGM and Proton), and PacBio sequencing.

The GSL provides Next Generation DNA sequencing, Sanger sequencing, genotyping and other support services to NC State faculty and the greater scientific research community.

The Genomics Core Facility provides DNA sequencing services for East Carolina University. The facility includes an Applied Biosystems 3130 Genetic Analyzer, Agilent 2100 Bioanalyzer, thermal cyclers, and Nanodrop 2000.

BTEC houses 63,000 GSF of fermentation, cell culture, recovery, purification, and analytical lab space for training NC State students and industry professionals in bioprocessing. BTEC also offers its space and expertise for protein production and purification, process/technology development, and analytical testing/development, serving both industry and other academic labs. Pre-clinical processing for non-human use only.

The UNC HTPSA Facility (part of the UNC Center for Structural Biology) provides researchers with high quality services for peptide synthesis, purification, and characterization of synthetic peptides. We specialize in synthesis of multiply modified peptides containing PTMs, unnatural amino acids, and fluorescent tags and peptide hydrazides for Native Chemical Ligation reactions.

The mission of the Histology Research Core Facility is to provide high-quality, cost-effective and timely histological results to researchers in the UNC system and abroad. This includes performance of existing, and development of new methodology for histological processing, sectioning and staining of both plant and animal specimens and all immunohistochemistry procedures. Other services provided: nissl, x-gal, beta gal, sectioning, staining, counterstain, prussian blue, cresyl violet, sudan iv

The Hooker Imaging Core (HIC) is an open core providing standard and advanced light microscopy, electron microscopy, and image processing resources, including live cell/tissue, confocal and super resolution. Assistance is provided to enable users to acquire images from samples they have prepared and provides electron microscopy sample preparation services. The HIC’s flagship confocal systems are a Leica STELLARIS 8 FALCON STED with White Light Laser for broad spectral range, fluorescence lifetime imaging and STED super-resolution, a Zeiss LSM 880 with Airyscan FAST for extra resolution, sensitivity and speed and an upright Zeiss 800 confocal. Additionaly, the HIC has systems for long-term live cell imaging (VivaView), high content image screening (GE IN Cell Analyzer 2200), automated slide scanning (Evident Scientific VS-200), and wide field systems for color and fluorescence imaging. Training and other assistance will be provided by the core staff. The Faculty Advisor is Dr. Stephanie Gupton.

This state-of-the-art laboratory is designed to perturb, measure, and analyze human movement, primarily gait. The centerpieces of the lab are a 3D motion capture system, and dual-belt instrumented treadmill.

State-of-the-art suite of charged particle, scanning probe and optical microscopes, including Helium Ion Microscope, Crossbeam SEM/FIB with EDX, TEM, VP-SEM (Environmental) with EDX, FE-SEM, Atomic Force Microscope, Confocal and Hyperspectral/Dark-field Microscopes.

Suite of spectroscopic and chromatographic characterization instruments including X-ray Photoelectron Spectrometer (XPS), 400 and 700 MHz NMR, GC Ion Trap Mass Spectrometer, GC/LC Triple Quad Mass Spectrometer, LC/MS Ion Trap Mass Spectrometer, HPLC Liquid Chromatography System, X-Ray Diffractometer, Confocal Raman Confocal Microscope, UV-Vis Spectrophotometer, FTIR Spectrometer w/ATR, ICP-OES Axial Spectrometer, ICP Mass Spectrometer, Fluorescence Spectrophotometer, Zetasizer and Nanoparticle Tracking and Goniometer/Tensiometer.