Shared Research Facilities and Equipment

The Proteomics and Metabolomics Shared Resource provides broad mass spectrometry services to academic and industry investigators. The PMSR mission is to provide access to advanced mass spectrometry techniques for quantitation and identification of proteins, metabolites and other molecules of interest in biological and clinical samples. In addition to the fee-for-service, the Shared Resource offers options to accommodate various needs such as training and project-based collaborations. All services are scheduled and managed through iLab https://wfbmc.ilabsolutions.com/account/login).

This is a user-operated facility that allows investigators to micro-dissect from tissue samples specific subsets of cells or sub-cellular structures, which usually are subjected to further protein or nucleic acid analyses. Users are required to undergo training.

The CMS DNA Analysis Core Facility provides UNCW faculty, students and researchers with the state-of-the-art infrastructure and expertise needed to generate and analyze DNA data for research and education.

Fermentation: bench to pilot scale We will provide affordable, practical hands-on training on bioreactor set-up, operation and harvest. Also, we will grow biomass (non-cGMP) for customers.

The NMR Facility houses a JEOL 400MHz spectrometer with a 24 position sample changer system and a JEOL 500MHz spectrometer. Both normal geometry and capillary NMR probes are available for small molecule elucidation and high-throughput screening.

The North Carolina State University Mass Spectrometry Facility (NCSU MSF) offers a variety of mass spectrometers providing analysis through an array of methods for sample introduction, ionization and mass measurement. Our mass spectrometers include the following: Thermo Fisher Scientific LTQ Orbitrap XL, Agilent Technologies 6210 LC-TOF, Agilent Technologies 5975 GC/MS, Thermo Fisher Scientific TSQ Quantum Discovery MAX Triple Quadrupole MS, Agilent Technologies GCD and AB Sciex 5800 MALDI-TOF/TOF MS.

This core facility offers high-resolution imaging of biological samples, including fluorescence microscopy, confocal high content imaging, and Widefield. It also features live cell imaging in high throughput format and calcium influx-efflux measurement using FLIPER.

The drug discovery core facility is a state-of-the-art high throughput screening (HTS) facility with over 450,000 compound small molecule chemical libraries. It specializes in assay development, HTS, medicinal chemistry and chemo-informatics for drug discovery.

Our mission is to help YOU with YOUR research. Electron microscopy services include project consultation, sample preparation, imaging and training on transmission and scanning electron microscopes. Light microscopy services include project consultation and training on image acquisition. Confocal, light-sheet, widefield fluorescence, darkfield, and polarization microscopy available. Image analysis services including training on image analysis (3D and 2D analysis, colocalization, macros, etc.)

Our mission is to provide surgical, imaging, and functional assessment services with a focus on the cardiovascular system in rodents. The core facilitates the assessment of cardiovascular function in rodents and works with all interested investigators to develop new models of cardiovascular disease or modify existing protocols. Our services include animal model microsurgeries (e.g., myocardial infarctions, ischemia reperfusions, pressure overload, implantation and cannulation, artery ligation, and models of thrombosis, stroke, and cardiac arrest). We also can perform electrocardiography, pressure-volume loops, cardiomyocyte isolation, and Lagendorff and working heart perfusions. There are also self-service equipment offerings, which include ultrasound, microsurgical work station rental, non-invasive blood pressure monitoring, and intravital confocal microscopy. Our director will meet with interested parties to collaborate on study design model selection, and data acquisition.