Shared Research Facilities and Equipment

The North Carolina State University Mass Spectrometry Facility (NCSU MSF) offers a variety of mass spectrometers providing analysis through an array of methods for sample introduction, ionization and mass measurement. Our mass spectrometers include the following: Thermo Fisher Scientific LTQ Orbitrap XL, Agilent Technologies 6210 LC-TOF, Agilent Technologies 5975 GC/MS, Thermo Fisher Scientific TSQ Quantum Discovery MAX Triple Quadrupole MS, Agilent Technologies GCD and AB Sciex 5800 MALDI-TOF/TOF MS.

The overall goal of the Choline & Metabolites Lab Facility is to provide cutting edge nutritional biochemical analysis of choline and metabolites in human, plant and mouse populations. To request choline & metabolites services, please complete our online service request form. [http://norc.sph.unc.edu/core_c/request_form.php]

METRIC is focused on advanced molecular characterization and is one of five NC State Shared Core Research Facilities administered by the Office of Research and Innovation. We offer technical expertise and state-of-the-art instrumentation encompassing magnetic resonance spectroscopy, mass spectrometry, and X-ray crystallography.

Our mission is to provide surgical, imaging, and functional assessment services with a focus on the cardiovascular system in rodents. The core facilitates the assessment of cardiovascular function in rodents and works with all interested investigators to develop new models of cardiovascular disease or modify existing protocols. Our services include animal model microsurgeries (e.g., myocardial infarctions, ischemia reperfusions, pressure overload, implantation and cannulation, artery ligation, and models of thrombosis, stroke, and cardiac arrest). We also can perform electrocardiography, pressure-volume loops, cardiomyocyte isolation, and Lagendorff and working heart perfusions. There are also self-service equipment offerings, which include ultrasound, microsurgical work station rental, non-invasive blood pressure monitoring, and intravital confocal microscopy. Our director will meet with interested parties to collaborate on study design model selection, and data acquisition.

Our mission is to help YOU with YOUR research. Electron microscopy services include project consultation, sample preparation, imaging and training on transmission and scanning electron microscopes. Light microscopy services include project consultation and training on image acquisition. Confocal, light-sheet, widefield fluorescence, darkfield, and polarization microscopy available. Image analysis services including training on image analysis (3D and 2D analysis, colocalization, macros, etc.)

Molecular genetics analysis including genotyping of populations for molecular markers analysis, QTL analysis and marker-assisted selection. The real-time PCR can be used for gene expression analysis. All investigators working at Mountain Horticultural Crops Research and Extension Center (MHCREC) will have free access to use any of these equipment for their research. However, they will have to schedule their time in coordination with the technician from the tomato breeding program. Investigators from outside the MHCREC are also encouraged to use the research facility whenever necessary. All users are expected to contribute supplies, particularly QIAxcel cartridge, micro plates, grinding beads, and tubes.

The laboratory provides instrumentation and assistance for multi-parameter flow cytometric analysis and sorting. It is available for use to all NCSU researchers and to those outside the NCSU community and operates as a by appointment, fee for service lab.

The NMR Facility houses a JEOL 400MHz spectrometer with a 24 position sample changer system and a JEOL 500MHz spectrometer. Both normal geometry and capillary NMR probes are available for small molecule elucidation and high-throughput screening.

The Pathology Services Core is a core facility in the UNC School of Medicine administered by the Lineberger Comprehensive Cancer Center and the Department of Pathology and Laboratory Medicine. We offer access to sophisticated digital pathology platforms and study design support to all UNC investigators to enhance translational research. Services are also open to users from other academic institutions and industry.

Animal imaging and hyperthermia treatment analysis consisting of a Quantum Design, Inc., DM100 series magnetic hyperthermia instrument and K-Laser Cube infrared laser system.