Shared Research Facilities and Equipment

SMIF provides researchers with access to advanced imaging, characterization and clean room fabrication capabilities. SMIF is available to all Duke University researchers and to external users from other universities, government labs, and industry

The TMS Laboratory provides mass spectrometry analysis and training to the UNC-Greensboro campus community and the surrounding Piedmont Triad. Our instrumentation can be tailored to small molecule, metabolomics, and/or protein/peptide analyses. Ionization Sources: ESI, APPI, APCI, MALDI, DESI Separation Instrumentation: Waters Aquity UPLC, Agilent 1100 HPLC Open to scientific researchers in the Piedmont Triad on a fee for service basis who are in need of mass spectrometry analysis. (Please see website http:triadmslab.uncg.edu)

The Advanced Analytics Core (AAC) provides support services for epithelial organoid research and high-quality, quantitative solutions for studying nucleic acids at both the bulk and single-cell level. The Core offers a wide variety of services, including: single cell -omics (10x Genomics/Fluidigm C1 scRNA-Seq and scATAC-Seq), high-throughput RNA-seq (Fluidigm Advanta), high-throughput qPCR/dPCR (Fluidigm BioMark HD), cell sorting (Sony SH800), spatial transcriptomics (10x Genomics Visium), library preparation (both manual and automated), sequencing (Illumina NextSeq 1000), ready-made conditioned media, and 2D/3D organoid culture support.

The Animal Metabolism Phenotyping Core provides contemporary phenotyping techniques for metabolism and energy balance in mouse models of obesity and nutritionally relevant disease. With locations at both the UNC Chapel Hill and UNC Nutrition Research;Institute campuses, the Core provides access to state-of-the-art methods, equipment, and populations to support high quality and high throughput phenotyping of energy balance components in mice.

The UNC Animal Models Core provides custom genetically modified mice, rats and related services. Services are open to both UNC and non-UNC clients. Available services include model design, custom nuclease design (CRISPR/Cas), vector construction, gene targeting, ES cell screening, ES cell microinjection, transgene or nuclease pronuclear injection and model characterization.

The Biomarker Mass Spectrometry Facility (BMSF) provides critical analytical support to UNC researchers for qualitative and quantitative assessment of environmental contaminants and biomarkers using mass spectrometry. The BMSF houses an AB Sciex 6500 mass spectrometer, Thermo TSQ mass spectrometers, Agilent QTOF, Agilent 8900 ICP-MS, Agilent 8860/5977C GC-MS and UPLC/nano-LC systems. Areas of focus for the BMSF include characterization of chemical reaction products, identification and quantitation of metabolites, quantitation of environmental chemicals, drug metabolism, measurement of DNA adducts, multi-element analysis and biomarkers of exposure and diseases in numerous environmental and biological matrices.

The BMSF has over 20 years of experience in developing new mass spectrometry methods, analyzing samples and training users to support investigators across UNC campus and beyond. We have longstanding collaborations and partnerships with UNC research Centers and Programs, including the UNC Superfund Research Program and UNC Center for Environmental Health and Susceptibility. The BMSF is proud to provide scientific and technical expertise throughout all stages of the customer service cycle. We look forward to working with you!