Shared Research Facilities and Equipment

The Cellular and Molecular Imaging Facility at North Carolina State University is located in 4115 Gardner Hall (North Campus) and contains a number of state-of-the-art light microscopes, including a Zeiss LSM 710 confocal. CMIF primarily serves the NC State community and is also open to scientists from other universities and local industries. The rates posted on our website are for NCSU students, faculty and staff. Please enquire about rates for facility users outside NCSU. Training and imaging assistance is available.

The Proteomics Core facility provides services for the analysis of proteins from tissues, cells or other biological samples. We are available to the UNC community, as well as external customers worldwide. We're committed to educating students and researchers in the field of proteomics and will work with you from initial experimental design through publication. We offer an array of sample preparation and instrumentation services, as well as method development and in-depth consultation to help researchers optimize their experimental design. The facility is equipped with three state-of-the-art systems: a Sciex 5800 MALDI-TOF/TOF, a Thermo Easy nLC 1000- QExactive HF, and a Waters nanoAcquity-Thermo LTQ-Orbitrap Velos. We offer several services including: protein identification, protein characterization, phosphoproteomics, large-scale proteomic profiling, and protein-protein interaction analyses.

The Crystallography and Computational Biosciences Core provide access to cutting-edge macromolecular X-ray diffraction as well as computational modeling and simulation methods.

The DHMRI bridges the research gap between academia and industry by providing applied R&D solutions for agriculture, nutrition, and health researchers in a customer-based, flexible, and collaborative environment. DHMRI applies a trans-disciplinary approach to solve complex biological problems. Areas of concentration include: Metabolomics, Proteomics, Genomics, Immunology, Cell and Biochemistry, and Bioinformatics. The DHMRI also maintains a state-of-the-art microscope suite which includes a Confocal Laser Scanning Microscope 710, Multiphoton NLO, a PALM Laser Microdissection system as well as several other microscope platforms. In addition, the facility maintains a 40k sq. foot AALAC-approved vivarium for small and large animal work. Collaborators range from academia to start-ups to Fortune 100 companies. Flexible business model from fee for service to shared risk.

The UNC Department of Chemistry Chemical Research Instrumentation Teaching and Core Laboratories (CRITCL) Mass Spectrometry core laboratory specializes in small molecule analysis. Our services include, but are not limited to, quantitative mass spectrometry, liquid chromatography separations, fragmentation and structural elucidation (MS/MS, MSn) mass spectrometry, complex mixture analysis, molecular formula confirmation (high resolution and accurate mass) analysis, & trace metal determination/quantitation. The mass spectrometry facility provides individualized user training for undergraduate, graduate, and post-docotoral scholars of the Department of Chemistry. In addition, the facility aims to provide the highest quality mass spectrometric services possible to members of the UNC Chapel Hill Department of Chemistry, UNC campus community and surrounding Research Triangle areas.

XCL primarily functions as a service crystallographic facility for the University and are actively collaborating with Department of Chemistry, Inorganic, Organic, and Material Division, Physics and Astronomy, biology, as well as the School of Pharmacy and the School of Medicine. The facility houses a single Crystal X-ray diffractometer, Bruker SMART Apex II, which is fully maintained by the XCL Director. Service includes SC-XRD structure determination and various PXRD techniques, and user training is available for both SC- and powder XRD techniques. Additional experiments at Argonne National Laboratory, SCrAPS – Synchrotron Crystallography at Advanced Photon Source, are carried out three times a year for samples that diffract too weakly using the conventional radiation source at the facility. XCL provides both client- and user-based services where clients enjoy the convenience of the service, and users gain privilege and flexibilities in instrument operation, data collection, and structure solution and refinement with or without the assistance of the XCL personnel. To become a proficient user, requirements such as passing the X-ray Crystallography course as well as instrument-specific and safety training and exams need to be fulfilled.

The Immune Profiling Core offers fee for service sample processing, cryopreservation, biobanking, and immunological assays such as flow cytometry, luminex, ELISpot, and ELISA. In addition, Core staff offer guidance in support of grant/contract applications as well as assay/study design, training and mentoring.

We provide light microscopy, laser scanning confocal microscopy, and electron microscopy (TEM & SEM) equipment and technical support to ASU and western North Carolina. See policy at: http://www.casmifa.appstate.edu/Policies.html

The Duke Chemistry Instrumentation Facility houses modern and diverse instrumentation for a range of analytical needs, including mass spectrometry, nuclear magnetic resonance, and a variety of spectroscopies. Most instruments in the instrument laboratories are available for researchers on a direct-walk-up basis once they have received the required training from the Facility’s director. Reservations for many instruments can be made on-line by a qualified user once they have been provided access to the calendars by the Facility’s director.

The DFGSR provides infrastructure and expertise for the implementation of CRISPR and RNAi-based technologies, both small- and genome-scale. We also provide compound screening services and high-throughput assay development. Open to non-Duke investigators.