Cell Culture Engineering: A Single-Use Perspective Course
In this three-day course, you will gain a fundamental understanding of the growth and
production of CHO cells. You'll get hands-on experience with a bench-scale stirred tank bioreactor, a rocker-bag bioreactor, and a 50-L single-use bioreactor. You'll learn about cell culture media, inoculum, cell banking, GMP requirements for upstream mammalian cell culture, scale up, single-use bioreactor versus stainless steel bioreactor design, and the use of DOE and data analysis in cell culture development.
Attend and you will learn
- Design of animal cell culture bioreactors, including suspension and immobilized cell bioreactors, conventional stainless steel stirred tanks, and disposable (single-use) bioreactors
- Advantages and disadvantages of single-use technologies relative to multi-use equipment
- Pros and cons for bioreactor types (rocker, stirred tank, microcarrier) and strategies (batch, fed-batch, perfusion)
- Setup and operation of single-use options for upstream manufacturing (rocker, stirred tank and perfusion)
- Setup and operation of a stainless steel bioreactor
- Advanced automation and control of animal cell culture processes, including bioreactor PAT
- Basic cost calculations to compare single-use options to more traditional multi-use set ups, to provide justification for single-use technologies
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NC State University
Centennial Campus
850 Oval Drive
Raleigh NC, 27695
$2,500