NCCU's Julius L. Chambers Biomedical/Biotechnology Research Institute
Located in Durham, North Carolina, North Carolina Central University is in the heart of the Research Triangle region, an area with a robust infrastructure for Biotech and Life Sciences.
The University is a comprehensive one offering programs at the undergraduate, graduate, and first-professional degree levels to nearly 9,000 students, is a constituent campus of the University of North Carolina.
Research Technician
Description:
This time-limited position is located in the Biomedical/Biotechnology Research Institute. The primary purpose of this position is to support and carry out cardiometabolic disease research in Dr. Ghosh’s laboratory. Dr. Ghosh is a new investigator and needs to generate preliminary data for grant submissions to the NIH and for subsequent studies leading to publications. A competent research technician will be able to carry out the experimental protocols leading to the generation of data while Dr. Ghosh can concentrate on grant submissions, data analysis and manuscript submissions. This arrangement will facilitate the establishment of a successful research program in cardiometabolic disorders at NCCU. The essential duties and responsibilities are as follows: 1. Ordering, receiving and maintaining inventory for laboratory supplies and equipments. 2. Evaluating, optimizing and trouble-shooting experimental protocols. 3. Executing project-related experimental protocols in cell-culture, animal models and molecular biology as detailed by Dr. Ghosh. 4. Generating, recording and storing data electronically.
Qualifications:
The successful candidate should have the following competencies: Knowledge: Basic knowledge about anatomy in mammalian systems, general biology knowledge, knowledge about molecular biology (DNA, RNA, PCR etc.) is needed. Must be able to understand and follow experimental protocols. Basic data analysis knowledge (simple statistics) needed. Knowledge about metabolic disease (obesity, diabetes) is a plus. Skills & Abilities: Cell culture – required skills in the areas of sterile techniques, preparing cell culture media, passaging cells, counting cells, cell viability and cell-based assays. Experience with NIH3T3 cells and primary cells, adipocyte differentiation, cellular glucose uptake, cellular RNA interference a plus. Genomics & Molecular biology – required skills in the areas of PCR, RT-PCR, DNA/RNA isolation, ELISA assays, nucleic acid and protein gel electrophoresis, Western blotting. Experience in gene cloning, genotyping a plus. Animal Studies – required skills in the areas of isolation of tissues (liver, muscle, adipose, kidney, etc.) from rodents, care and experimental treatment of rodents and drawing small volumes of blood. Previous experience with working with mice tongues is a plus. Data Management – required skills in the areas of entering data in spreadsheets, organizing data and formatting data for downstream analysis. Ability to apply basic statistical tests to data a plus. A Bachelor’s Degree in a discipline related to the area of this assignment is required with a combination of training and experience. All degrees must be received from appropriately accredited institutions.
Contact Information:
Connie
Key
NCCU's Julius L. Chambers Biomedical/Biotechnology Research Institute
1801 Fayetteville St.
Durham, NC 27707
Fax: 919-530-7998
Posted January 12, 2010.